INTENSITY OF TISSUE RESPIRATION AND OXIDATIVE PHOSPHORYLATION IN MITOCHONDRIA OF HEPATOCYTES OF RAT UNDER THE INFLUENCE OF SODIUM FLUORIDE

PUMTS. 2019; 35(3): 57-61.
https://doi.org/10.31071/promedosvity2019.03.057

Mature rats of the Wistar population (N = 24), who were daily injected intravenously with aqueous solutions of sodium fluoride at a rate of 20 mg / kg animal weight, taking into account 200 mg / kg LD50, the duration of lowdose subtoxic infections – for 1.5 months, were studied pathophysiology mechanisms intoxication. Metabolic state of mitochondria after determination of the rate of oxygen consumption in the non-receptor medium (V4), the rate of oxygen consumption in the presence of the acceptor (V3), the rate of oxygen consumption after exhaustion, add th ADP in the presence of the 2,4-dinitrophenol (2,4-DNF disconnector) ) (V4P) was significantly reduced. The calculation of the ratio of ADP / O2, similar in value to the coefficient of phosphorylation of P / O, which characterizes the conjugation of oxidation and phosphorylation processes in the respiratory chain is also clearly and significantly reduced; respiratory factor (DC) – the ratio of the rate of oxygen absorption in the state V3k absorption rate in the state V4 (before the introduction of ADP) and the activity of ATP-hydrolases reactions as the ratio V4 / V4P, characterizing the rate of regeneration of ADP after its phosphorylation. Measurement of ATPase activity (Ca2 + – and Mg2 + – dependent ATPase), which was calculated in μmol of phosphate / 1 mg of protein per hour, showed a decrease. Sodium fluoride at a subtoxic dose of 20 mg / kg animal weight (1/10 LD50) inhibits and separates the processes of tissue respiration and oxidative phosphorylation, which reveals the mechanism of fluoride (small doses) of experimental chronic intoxication.

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